Fig. 3. Ethanol induced transcriptome and proteome perturbations in ethanol-treated myotubes and mice with alcohol-related liver disease. (A). Heatmap of the transcriptome and proteome of the ubiquitination pathway in ethanol treated myotubes and muscle from mALD. (B). g:Profiler results for RNAseq data from differentially upregulated genes in 6h EtOH treated C2C12 myotubes compared to untreated myotubes. (C). Venn diagram of differentially expressed unique and shared HMB target genes on the transcriptome and proteome in ethanol-treated myotubes at different timepoints aligned with genes involved in sarcopenia on OpenTarget. (D). Differentially expressed genes regulating muscle protein homeostasis pathways in the transcriptome of mALD.